AMYLOID PRECURS PROTEIN; RB-100UL

Application

Research Sub CategoryNeurodegenerative Diseases

Research CategoryNeuroscience

Western blot: 1:250 - 1:1,000: need to reduce and alkylate disulfide bonds of APP to expose the epitope on APP transferred to nitrocellulose or PVDF prior to immunoblotting.

To reduce incubate the slide (Blot) in TBS (PBS) + 50mM 2-mercaptoethanol at 37°C for 3 h in the dark. Wash twice with TBS, then incubate in TBS (PBS) + 20 mg/ ml of Iodoacetic acid for 30 min at room temperature. Wash twice with TBS (PBS), block and process as usual.

Immunoprecipitation: Need to reduce and alkylate disulfide bonds to expose the epitope

Optimal working dilutions must be determined by end user.

This Anti-Amyloid Precursor Protein Antibody, universal is validated for use in IP, WB for the detection of Amyloid Precursor Protein.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

APP (aa 99-126) conjugated to KLH.

Epitope: universal

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Serum. Liquid in PBS with 0.01% azide.

Specificity

Amyloid Precursor Protein (APP), Universal. Specific to amino acids 99-126.

Storage and Stability

Maintain frozen at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.